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Free Webinar : Multiplexing Mass Spectrometry-Based Identification

About Webinar:
The identification and quantitative measurement of different forms of protein site-specific reversible cysteine modifications remain technically challenging. Many mass spectrometry (MS)-based workflows have been developed, all sharing a common principle of first blocking any free thiol before labeling the specific forms of cysteine modifications that can be reversed by specific reducing agents. In essence, this “switches” the reversibly modified cysteine into chemically labeled cysteine more suitable for MS-based detection and quantification. The recent availability of multiplexed iodoTMT, a set of isobaric mass tags that irreversibly label Cys-thiols, now facilitates determination of the relative degree of specific cysteine modifications under different redox conditions. In this webinar, Dr. Kay-Hooi Khoo (Academia Sinica, Taiwan) will describe how multiplexed iodoTMT has been used to sequentially and differentially tag S-nitrosylation and S-glutathionylation, allowing their dynamics to be delineatedDr. Khoo will answer questions from webinar attendees so be sure to attend and get answers from an expert!
Date & Time:
Sept 30, 2014 (Thursday) 23:00 (GMT+8)
Click here to check it in your timezone.
What you will learn:
  • Understanding the complexity of cysteine modifications
  • Advantages of using iodoTMT in a sequential labeling workflow
  • Potential applications to delineate hypoxia-induced redox events
Who should attend?
  • Researchers using MS-based proteomics techniques for analysis of post-translational modifications
  • Scientists interested in using the technology to probe redox-mediated cysteine modifications
About the speakers:
  • Dr. Kay-Hooi Khoo received his Ph.D. in Biochemistry from Imperial College, London. He then stayed at Imperial College as a Wellcome Trust Prize Postdoctoral Fellow for another year before moving to Department of Microbiology, Colorado State University as a Heiser Fellow for Research in Leprosy and Tuberculosis and worked there for another two years. He started his independent research career at the Institute of Biological Chemistry, Academia Sinica in Taiwan and currently holds the title of Distinguished Research Fellow and serves as Associate Director. He is also the Chair of Mass Spectrometry Committee at Academia Sinica, overseeing the running of various common MS facilities at several affiliated institutes.Dr. Khoo’s research interests are focused primarily on the development and applications of concerted mass spectrometry-based analytical techniques for high sensitivity structural mapping and sequencing of complex glycans in the context of targeted and systematic glycomics. Additionally, he is actively involved in using advanced shotgun proteomics technology to define a diverse range of site-specific protein modifications, with special interest in cysteine modification, particularly the roles of S-nitrosylation of protein tyrosine phosphatases in redox-modulated signaling events.
How to register:
  • Click here to sign up

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